Modulation of virulence factors of Staphylococcus aureus by nanostructured surfaces

Investigating and understanding the response of microbes to various surfaces requires a versatile parametrisation surface, multiple assays that captures complexity biofilm structures. Here, Staphylococcus aureus viability, polysaccharide poly-N-acetylglucosamine, proteins on cell surface were analysed with agar plate- well plate-based formation assays. Biofilms grown set nanostructured polymeric surfaces, which thoroughly characterised for their chemistry topography. Surface hydrophobicity, summit density as peak valley structure found influence microbial viability exopolysaccharide abundance level in plate assay. In assay, chemical parameters had lesser but roughness caused by structures increased decreased expression. relating pathogenicity affected The profile these correlated clearly several parameters, especially fine assay lateral These results highlight necessity describing material different completely understand what specific features drive certain bacterial response.